![]() Applying our approach to yellow fever vaccination as a model of acute infection in humans, we identify candidate clones participating in the response.Ĭitation: Puelma Touzel M, Walczak AM, Mora T (2020) Inferring the immune response from repertoire sequencing. ![]() We then use that null model as a baseline to infer a model of clonal expansion from two repertoire time points taken before and after an immune challenge. Using replicate experiments, we first show how to learn the natural variability of read counts by inferring the distributions of clone sizes as well as an explicit noise model relating true frequencies of clones to their read count. Here, we present a general Bayesian approach to disentangle repertoire variations from these stochastic effects. However, quantitative comparison between repertoires is confounded by variability in the read count of each receptor clonotype due to sampling, library preparation, and expression noise. Benchmarking our method on co-extracted DNA with amplicon-based repertoire sequencing confirmed accurate detection of highly abundant TIL receptors using an orthogonal approach.Ĭonclusions: Our repertoire profiling method effectively leveraged routine RNA-seq to profile TIL receptors, ultimately allowing for the seamless integration of repertoire-based biomarker evaluation and improved immune infiltrate estimation through a high-volume RNA-seq pipeline.High-throughput sequencing of B- and T-cell receptors makes it possible to track immune repertoires across time, in different tissues, and in acute and chronic diseases or in healthy individuals. Repertoire richness was significantly correlated with RNA-based cell-type-specific transcriptional profiles for T-cells (r = 0.43, P<5e-24) and B-cells (r = 0.2, P<3.6e-6). Repertoire richness also followed tissue-specific expectations where TIL-low cancers (e.g., glioblastoma multiforme) typically exhibited low-richness repertoires, while TIL-high cancers (e.g., non-small cell lung cancer) often yielded thousands of productive clonotypes. The distribution of repertoire evenness demonstrated expected trends, including dominant monoclonal expansion (>70-90% total receptor reads) of putative tumor cell receptors in T-cell/B-cell-driven malignancies. TCR and BCR repertoires (including small gamma/delta TCR repertoires) were robustly assembled in our cohort, with over 1.2 million total clonotypes identified. Results: Immune repertoires were extracted from RNA-seq of 501 tumor samples across 38 different cancer types. Richness (productive clonotypes) and immune infiltration predictions were correlated with a one-tailed Pearson correlation coefficient (95% CI). ![]() Shannon Entropy was calculated to evaluate repertoire evenness. Receptor sequences were aligned, assembled, and annotated against IMGT reference sequences using the TRUST4 assembly tool. Methods: Hybrid capture probes tiling T-cell and B-cell receptor (TCR and BCR) genes were used to enrich immune receptor transcripts detected by the Tempus RNA-seq workflow. Here, we demonstrate the capabilities of our method in 500 TCR/BCR repertoires from a diverse cohort of tumor samples. Our approach yields accurate quantification of abundant TIL receptors without diminishing the clinical value of the remaining transcriptome. We developed and optimized a method to integrate immune repertoire profiling into a high-volume RNA-seq pipeline. However, repertoire profiling of the immune infiltrate is often implemented as a stand-alone assay or via single-cell sequencing, which can be difficult to incorporate into diagnostic platforms at scale. Accumulating evidence indicates that better characterizing TILs using repertoire-sequencing (rep-seq) may aid in predicting immunotherapy outcomes. ![]() Background: Therapeutic strategies that modulate the interaction between tumors and tumor-infiltrating lymphocytes (TILs), such as checkpoint blockade, have tremendous promise but yield clinical responses in only a subset of patients.
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![]() Due to the background being defocused, I could get away with using this simple method. Then I simply animated the stills of the models across the screen, then added motion blur. ![]() To complete the illusion, I photographed friends and co-workers on greenscreen and put them in the miniature cars as drivers. It saved us countless hours of having to render CG cars for the number of shots, and no one would be any the wiser if I had not mentioned it here. What is the Back to the Future Mod This mod (at the moment) adds in The Delorean and the Mattel Pink / PitBull Hoverboards and (Pepsi Perfect (1.5. Some people might be wary of using such a low-tech solution in a film, but if you can get away with it, and it looks good, then there's no reason not to. Back to the Future - Roads Clock By MillzyMerch 35.59 DeLorean Clock By izacless 37.01 Twin Pines Mall Clock By D24designs 35.59 Back to the Future - Delorean Time Machine Clock By AbidingCharm 34.16 Back To The Future Clock By joshbaum 34.16 BACK TO THE FUTURE 88 MPH Clock By KoolDsigns-FLIX 34. We put the cars on a lazy susan so that we could move the models around to get the angle we wanted, instead of having to move the camera around. We shot outdoors in natural sunlight to make sure it matched the natural light of the plate. And lastly, kudos to Bricksonville on YouTube for giving me the first spark to design this Modular and informing my overall approach to the building's color scheme.ĭimensions: approximately 10.1" x 20.2" x 12.To get the minatures in the shots, my buddy Jonathan and I simply photographed stills of the models against green or blue posterboard depending on the model's color. Delorean flying down main Street, doc hanging off the clock etc 3.An additional thanks to HP_Brixxter on LEGO Ideas and their amazing " Ancient Roman Temple" for inspiring my approach to the Corinthian columns' design. Find These Creators HereStridey JJtoxic123 DogeThaCop Rob Kindleyside Spark Minecraft LogicalGeekBoy RedPlasma Studios Music.(It's also a similar story with 2 x 4 white tiles.)ĭon't miss checking out all my nerdy MOC instructions I have available on Rebrickable, and you can also see all of my MOCs on Instagram ( My sincere thanks to " toutflou" on 3dWarehouse for their amazing 3D model of the Clock Tower, which proved to be an invaluable source of reference. 26 of this piece are needed for this MOC, so that could be a $5.46 savings. 87079 (4560184) 2 x 4 Tile (Dark Bluish Grey) - goes for $0.36 USD on Bricklink and it's currently $0.15 from LEGO directly. Clock tower (done in Pe survival) : Minecraft 5.4m members in the Minecraft community.You need 30 for this MOC, so that could be a potential savings of $7.20. 87087 (6251021) Brick with Stud on the side (Medium Nougat) - goes for $0.33 USD on Bricklink right now compare that to $0.09 on LEGO's site.High-quality Instructions for this MOC are split out across three easy-to-follow PDFs, and a separate PDF file lists out the required parts.Īside from acquiring pieces via Bricklink, it's also never a bad idea to take a look at LEGO's Pick a Brick site, especially when ordering some pieces in bulk. This leaves you with plenty of fun and opportunity to design your own look. ![]() To limit the piece count (and because the inside of the building was never meaningfully shown on-screen), the interior of this MOC is bare-bones but functional. A building as iconic as this one is sure to make the perfect addition to any “Back to the Future” fan’s LEGO city! It's enough to make any visitor exclaim, “Great Scott!” This double corner-style modular building (64 x 32 studs) was meticulously designed after the famous Hill Valley Courthouse & Clock Tower. ![]() ![]() Maybe Justin Bieber could get Diplo and Skrillex to do a slowed down vocoder version with a dubstep break – that would certainly be interesting." Bust a Move" is a song by British-American rapper Young MC from his 1989 debut album, Stone Cold Rhymin'. Who could sing this today and have a hit? Given Young’s superbly dry delivery on the original, it’s hard to imagine who could cover this without sounding ridiculous. Most importantly, his hit remains a reminder of that age when rap, in many different forms, moved straight outta the sidelines and straight into the pop mainstream. While Young only managed one more Top 40 hit in the US, he continues to record and has also achieved success on reality television and as an actor. In the UK, however, it spent a mere 5 weeks on the chart, crawling to a subpar no.73. The song also earned Young the Grammy for Best Rap Performance. You may have to hide your children and breakables, but you should be able to capture some superfly middle-aged dance moves on your iPhone).īust a Move topped the chart in Australia in late 1989 and reached number 7 in the US, where it also spent 39 weeks on the Hot 100, including 20 weeks in the Top 40. (Don’t believe me? Put it on at your next boring social event and watch what happens. Initially derided as a shallow novelty hit, Bust a Move is now considered a pop-rap classic and a true party-starter. Take this nugget from the middle verse (where the song’s hero is working his game in a movie theater): “Theater gets dark just to start the show / Then ya spot a fine woman sittin’ in your row / She’s dressed in yellow, she says “Hello / Come sit next to me you fine fellow” / You run over there without a second to lose, and what comes next? / Hey bust a move!” Stephen Sondheim eat your heart out, these are some classic turns of phrase, in a song chock full of them. Delivered by Young in a cool “i-won’t-break-a-sweat” baritone, they elevate the song out of monotonous rap hell and into pop-rap heaven. But the track’s not-so-secret weapon? The hilarious and quotable lyrics, among the wittiest and silliest in rap music history. The beats of the song – including samples from Scorpio by Dennis Coffey and Daytime Hustler by Bette Midler (!!!) – render it immediately dance-floor ready. First, the song’s musical track – based mainly on a sample of the song Found a Child by the group Ballin’ Jack – is accessible and irresistible. Ostensibly an ode to rejecting shyness and making one’s move with the lady of one’s choice, the song is a fantastic primer for everything that is wonderful about the pop-rap hits of this era. The first single from Stone Cold Rhymin’ was the great Bust a Move. This partnership set the stage for Young’s first album, Stone Cold Rhymin’, which was released in late 1989 and made the US Top 10. There, he met the rapper Tone Loc, and collaborated with him on the songs Wild Thing and Funky Cold Medina, which became top 5 pop chart hits and million sellers in the US. While a student at USC, Young was offered a record contract from the rap label Delicious Vinyl after rapping over the phone to label executives. Born in London and raised in Queens, New York, Young moved to LA in the late 80s to attend the University of Southern California where he earned a degree in economics. On the pop singles charts, rap as a genre didn’t really begin its big breakthrough until 19, when a diverse platform of both good and bad rap artists – Tone Loc, Monie Love, Vanilla Ice, Salt-n-Pepa, and MC Hammer among many others – stormed radio and the pop sales charts on both sides of the Atlantic and began to establish rap as the consistent chart hit generator that it remains to this day.Ĭaught up in this sweeping ’89-90 wave of pop-rap was one Marvin Young, otherwise known as Young MC. Sure, there were some rap songs that were big chart hits prior to that year – Rapper’s Delight by the Sugar Hill Gang, Walk this Way by Run DMC, The Message and White Lines by Grandmaster Flash, (You Gotta) Fight For Your Right (to Party) by the Beastie Boys and, erm, Holiday Rap by MC Miker G and DJ Sven, but these were mainly outliers. But truth be told, when the Straight Outta Compton album was released in 1988, rap music of any kind was only just beginning to make a dent on the upper reaches of the pop singles charts. 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She has a vision that is thoughtful in the sense that she’s not just thinking about today or tomorrow but years out.” ![]() She has a great wealth of experience not just in the museum world. Gary was the frontrunner from the beginning. ![]() “We were looking for the next executive director to continue the work Debi had started. “The museum has seen a great evolution over the last five years,” Virginia MOCA board chair Andrew Hodge told ARTnews. The museum has slowly gained a national reputation for staging well-regarded exhibitions, including solo outings by Inka Essenhigh in 2018 and Mickalene Thomas in 2017, and the 2017 group exhibitions “ Mindful: Exploring Mental Health Through Art,” and “ Southern Routes,” which featured work by photographers from throughout the American South. Under Gray’s tenure, Virginia MOCA received accreditation from the American Alliance of Museums in 2010 and rebranded its identity the following year. Between 19, Ryan held various positions at Time, Inc. Prior to joining the Katonah Museum, she founded Palermo Strategy, a fundraising consulting firm, and worked in development positions at the Metropolitan Opera in New York and the Brooklyn Academy of Music. She has been the museum’s development director since January, and served as its interim co-executive director between February and July. At Virginia MOCA, Cut to the Quick will be guest curated by Rouse with Virginia MOCA Senior Curator Heather Hakimzadeh.Ryan has worked at the Katonah Museum of Art in Upstate New York, about an hour north of Manhattan, since 2016. ![]() In addition to her curatorial responsibilities, Rouse composed original poems inspired by Walker’s works, which live inside the exhibition. This exhibition originated at the Frist Art Museum in Nashville, Tenn., and was co-curated by former Frist Art Museum executive director and CEO Dr. Intentionally unsentimental and ambiguous, the works can be disturbing while also utilizing satire and humor, always exploring the irreconcilable inconsistencies that mirror the human condition. Walker’s process involves extensive research in history, literature, art history, and popular culture. Some highlights of the exhibition are the complete Emancipation Approximation series and images from the Porgy & Bess series. Schnitzer and the Jordan Schnitzer Family Foundation, premier collectors of works on paper in the United States. This exhibition offers a broad overview of her career through more than 80 works from the collections of Jordan D. Her powerful and provocative images employ contradictions to critique the painful legacies of slavery, sexism, violence, imperialism, and other power structures, including those in the history and hierarchies of art and contemporary culture. 1969) works in a range of mediums, including prints, drawings, paintings, sculpture, film, and the large-scale silhouette cutouts for which she is perhaps most recognized. A leading artist of her generation, Kara Walker (b. In 2012 he was named CEO of the Year by the Greater Miami Chamber of Commerce, as well as Ultimate CEO by the South Florida Business Journal (SFBJ). The business community in South Florida has recognized Prendes’ entrepreneurial success multiple times in recent years. In just five years, Prendes turned the company into an award-winning leader in the professional-grade supplement world and one of the fastest growing web merchants in the U.S. He ventured into e-commerce in 2001, when he founded, a successful digital retailer of pet supplies and medications. in the 1990s to fulfill his American Dream. L-R: CIO William Perez, CEO Jose Prendes, CMO Daniel Moure, COO David Rodriguez.īorn and raised in Cuba, Prendes moved to the U.S. She also volunteered in the nutrition department of Baptist’s South Miami Hospital. Cavalcante will bring her expertise to our readers with a monthly column. During her time at FIU, she taught nutrition labs for the class Foundations of Nutrition Science for two years and worked as a wellness assistant at FIU’s Student Health Services. ![]() She has a bachelor of science in dietetics from one of the top schools in her native Brazil and a master of science in dietetics and nutrition from FIU. 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